离子缔合显色反应分光光度法测定克拉霉素的含量

doi:10.16597/j.cnki.issn.1002-154x.2026.02.005

离子缔合显色反应分光光度法测定克拉霉素的含量

成帅^1,2 董润泽^1,2 黄锦^1,2 刘岩^1,2 马卫兴^1,2∗

(1. 江苏海洋大学 药学院, 连云港 222005; 2. 江苏海洋大学生物医药产业学院, 连云港 222005)

摘 要 曙红Y是一种多卤代荧光素类阴离子染料,在pH为2.87的Britton-Robinson缓冲介质中,曙红Y阴离子与质子化的克拉霉素阳离子发生离子缔合显色反应形成红色的二元配合物,其最大吸收波长位于540 nm。作者通过条件优化建立了离子缔合显色反应分光光度测定克拉霉素的新方法,其工作曲线的线性回归方程为A₅₄₀ = 0.0109C + 0.0415(C的单位: mg·L^-1),相关系数R = 0.9991,克拉霉素的含量在2~40 mg·L^-1之间符合Beer定律呈现线性关系。所建立的方法成功地测定了克拉霉素样品中的克拉霉素含量且简单、快速。

关键词 克拉霉素　曙红Y　离子缔合显色反应　分光光度法

Determination of Clarithromycin Content by Ion-Association Complex Formation Reaction Spectrophotometry

CHENG Shuai^1,2 DONG Runze^1,2 HUANG Jin^1,2 LIU Yan^1,2 MA Weixing^1,2∗

(1. School of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China; 2. School of Biomedical Industry, Jiangsu Ocean University, Lianyungang 222005, China)

Abstract Eosin Y is a polyhalogenated fluorescein-type anionic dye. In a Britton-Robinson buffer medium at pH 2.87, the anionic eosin Y reacts with the protonated clarithromycin cation through an ion-association chromogenic reaction to form a red binary complex. The maximum absorption wavelength of this complex is at 540 nm. By optimizing the conditions, a new spectrophotometric method for determining clarithromycin based on the ion-association chromogenic reaction was established. The linear regression equation of the working curve is A₅₄₀ = 0.0109C + 0.0415 (where C is in mg·L^-1), with a correlation coefficient R = 0.9991. The content of clarithromycin, ranging from 2 to 40 mg·L^-1, conforms to Beer's law, showing a linear relationship. This method successfully determined the clarithromycin content in clarithromycin samples, and the proposed method was simple and rapid.

Keywords clarithromycin eosin Y ion-association chromogenic reaction spectrophotometry